Actions for Strand breaks, base release and post-irradiation changes in DNA. gamma. -irradiated in dilute O2-saturated aqueous solution

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Strand breaks, base release and post-irradiation changes in DNA. gamma. -irradiated in dilute O2-saturated aqueous solution

Author
Ward, J. F.
Published
United States : [publisher not identified], 1976.
[Oak Ridge, Tennessee] : [U.S. Atomic Energy Commission], 1976.
Physical Description
microfiche : negative ; 11 x 15 cm
Additional Creators
Guo, Yi

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Summary
Gamma irradiation of DNA in dilute O/sub 2/-saturated aqueous solution releases free bases and damaged bases from the macromolecule. The yields of these products were measured after column chromatographic separation. For double stranded DNA the immediate yield of bases varies from G = 0.012 for cytosine to G = 0.033 for adenine. The yields of released bases increase with post-irradiation time (the majority of the increase occurs in the first 2 hrs.) to yields in the range of G = 0.07 +- 0.012. Yields of two released damaged thymine radiation products from ..gamma..-irradiated /sup 3/H thymine labelled DNA also increased with post-irradiation time. Strand breaks were measured in ..gamma..-irradiated single stranded DNA the initial yield G = 0.02 was low but increased with time to G = 0.07. No direct correlation between strand-break production and release of low molecular weight products is possible. The findings are discussed in terms of damage to DNA in vivo and its enzymatic repair.
Report Numbers
UCLA-12-1060
Other Subject(s)
Collection
U.S. Atomic Energy Commission depository collection.
Note
DOE contract number: E(04-1)-GEN-12
OSTI Identifier 7363227
Research organization: California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology.
View MARC record | catkey: 47046596