Actions for Attempted induction of resistance to melphalan by pretreating Chinese hamster cells with subtoxic doses of the drug

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Attempted induction of resistance to melphalan by pretreating Chinese hamster cells with subtoxic doses of the drug

Author
Kaye, M. F.
Published
United States : [publisher not identified], 1984
Springfield, Va.: National Technical Information Service, [approximately 1984]
Physical Description
microfiche : negative ; 11 x 15 cm
Additional Creators
Tobey, R. A.

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Summary
By pretreating the line Chinese hamster (CHO) cells with multiple subtoxic doses of melphalan before challenge with a toxic dose, we established optimal conditions to increase the resistance of the cells to melphalan toxicity. Variables examined included: (1) level of drug used to induce protection (0.0008 to 0.8 ..mu..M); (2) interval between administration of successive low doses of drug (3 to 12 h); and (3) duration of induction period (24 to 96 h). The greatest degree of protection (an increase by 2 to 2.5 times in survival following challenge with 16 ..mu..M melphalan for 2 h) was achieved with a level of 0.08 ..mu..M melphalan administered every 6 h for 48 h. Combining the low-level drug induction protocol described above with our previously described trace element induction protocol (treatment with 125 ..mu..M CuCl2 for the last 9 h of the induction period) increased survival slightly over that achieved with either single-induction protocol. If we assume that the induction protocols yielded a slight but real degree of protection, it appears that the induced protection comprises a common component representing activities elicited by the two induction protocols and a component that represents protective processes that are unique to a given induction protocol. 10 references, 1 figure, 3 tables.
Report Numbers
DE85002342; LA-10176-MS
Other Subject(s)
Collection
NTIS collection.
Note
DOE contract number: W-7405-ENG-36
OSTI Identifier 6309445
Research organization: Los Alamos National Lab., NM (USA).
View MARC record | catkey: 47346881