Actions for Characterization of the heterokaryotic and vegetative diploid phases of Magnaporthe grisea

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Characterization of the heterokaryotic and vegetative diploid phases of Magnaporthe grisea

Author
Crawford, M. S.
Published
United States : [publisher not identified], 1984
Springfield, Va.: National Technical Information Service, [approximately 1984]
Physical Description
microfiche : negative ; 11 x 15 cm
Additional Creators
Chumley, F. G., Valent, B., and Weaver, C. G.

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Summary
The heterokaryotic and vegetative diploid phases of Magnaporthe grisea, a fungal pathogen of grasses, have been characterized. Hyphal tip cells and conidia (vegetative spores) taken from these heterokaryons are auxotrophs with phenotypes identical to one or the other of the parents. M. grisea heterokaryons have completely septate hyphae with a single nucleus per cell. Heterokaryons have been utilized for complementation and dominance testing of mutations that affect nutritional characteristics of the fungus. Heterokaryons growing on minimal medium spontaneously give rise to fast-growing sectors that have the genetic properties expected of unstable heterozygous diploids. In fast-growing sectors, most hyphal tip cells are unstable prototrophs. The conidia collected from fast-growing sectors include stable and unstable prototrophs, as well as auxotrophs that exhibit a wide range of phenotypes, including many recombinant classes. Genetic linkage in meiosis has been detected between two auxotrophic mutations that recombine in vegetatively growing unstable diploids. This suggests that homologous recombination occurs during vegetative growth of M. grisea. No interstain barriers to heterokaryosis and diploid formation have been detected. The mating type of the strains that are paired does not influence the formation of heterokaryons or diploids.
Report Numbers
DE86008528; DOE/ER/13160-3
Other Subject(s)
Collection
NTIS collection.
Note
DOE contract number: AC02-84ER13160
OSTI Identifier 5895608
Research organization: Colorado Univ., Boulder (USA). Dept. of Chemistry.
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