Protein chromatography [electronic resource] : methods and protocols / edited by Dermot Walls and Sinéad T. Loughran
- Springer protocols and Methods in molecular biology, 1940-6029 ; v. 681
- A digest of protein purification / Dermot Walls, Robert McGrath, and Sinéad T. Loughran -- Gel-filtration chromatography / Ciaran Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor -- Immunoaffinity chromatography Jenny Fitzgerald ... [et al.] -- Avoiding proteolysis during protein chromatography / Barry J. Ryan -- Scale-up of protein purification : downstream processing issues / John Joseph Milne -- Phage display : a powerful technology for the generation of high specificity affinity reagents from alternative immune sources / William J.J. Finlay, Laird Bloom, and Orla Cunningham -- Engineering protein stability / Ciarán Ó'Fagáin -- Microfluidics in protein chromatography / Frank A. Gomez -- Tagging recombinant proteins to enhance solubility and aid purification / Dermot Walls and Sinéad T. Loughran -- Storage and lyophilisation of pure proteins / Ciarán Ó'Fagáin -- Differential precipitation and solubilization of proteins / Barry J. Ryan -- Ion-exchange chromatography : basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase / Philip M. Cummins, Oonagh Dowling, and Brendan F. O'Connor -- Protein quantitation and analysis of purity / Eva M. Campion, Sinéad T. Loughran, and Dermot Walls -- Purification of proteins fused to glutathione S-transferase / Sandra Harper and David W. Speicher -- Purification of proteins fused to maltose-binding protein / Mario Lebendiker and Tsafi Danieli -- Purification of proteins from baculovirus-infected insect cells / Luke O'Shaughnessy and Sean Doyle -- Purification of poly-histidine-tagged proteins / Sinéad T. Loughran and Dermot Walls -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by CID/ETD tandem mass spectrometry / Rosana Navajas, Alberto Paradela, and Juan Pablo Albar -- Tag removal by site-specific cleavage of recombinant fusion proteins / Adam Charlton and Michael Zachariou -- Purification of antibodies using affinity chromatography / Elaine Darcy ... [et al.] -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens / William J.J. Finlay, Laird Bloom, and Orla Cunningham -- Measuring protein-protein interactions using Biacore / Paul Leonard, Stephen Hearty, and Richard O'Kennedy -- Ultra-performance liquid chromatography-mass spectrometry of proteins / Timothy R. Croley -- Hydrophobic interaction chromatography / Philip M. Cummins and Brendan F. O'Connor -- Fast protein liquid chromatography / Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan -- Shotgun proteomics : a qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS / Laurent Geiser ... [et al.] -- Shotgun proteomics : a relative quantitative approach using off-gel electrophoresis and LC-MS/MS / Laurent Geiser ... [et al.] -- Clinical proteomics : liquid chromatography-mass spectrometry purification systems / Michael Henry and Paula Meleady -- Strategies for the purification of membrane proteins / Sinead Marian Smith -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases / Tracey McCarthy and Maria G. Tuohy.
- 9781607619123 (alk. paper) and 1607619121 (alk. paper)
- Bibliography Note:
- Includes bibliographical references and index.
- Source of Acquisition:
- UP-PAT copy: Purchased with funds from the James E. Wright, Jr. Memorial Libraries Collections Endowment; 2010.
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