Cryo-EM. Part B, Reconstruction [electronic resource] / edited by Grant J. Jensen
- Published
- San Diego, Calif : Academic Press/Elsevier, [2010]
- Copyright Date
- ©2010
- Physical Description
- 1 online resource (xlv, 410 pages) : illustrations
- Additional Creators
- Jensen, Grant J.
Access Online
- Series
- Contents
- Machine generated contents note: Section I UPR and Cell Stress in Normal and Diseased Cells -- 1.The Unfolded Protein Response in Mouse Cerebral Cortex / Nirinjini Naidoo -- 1.Introduction: The UPR -- 2.Transduction of the UPR Signal by PERK, IRE1, and ATF6 -- 3.UPR and Apoptosis -- 4.The UPR in Health and Disease -- 5.Induction of the UPR by Sleep Deprivation -- 6.Experimental Protocols -- 7.Concluding Remarks -- Acknowledgments -- References -- 2.Immunohistochemical Detection of the Unfolded Protein Response in Atherosclerotic Plaques / Richard C. Austin -- 1.Endoplasmic Reticulum and the Unfolded Protein Response -- 2.Atherosclerosis -- 3.UPR in the Atherosclerotic Lesion -- 4.Mouse Models of Atherosclerosis -- 5.Methods -- Acknowledgments -- References -- 3.The Role of PDI as a Survival Factor in Cardiomyocyte Ischemia / Alfonso Baldi -- 1.Introduction -- 2.Identification of PDI as Cardio-Protective Factor -- 3.Conclusions -- References -- 4.Measurement of the Increase in Endoplasmic Reticulum Stress-Related Proteins and Genes in Adipose Tissue of Obese, Insulin-Resistant Individuals / Salim Merali -- 1.Introduction -- 2.Study Subjects and Fat Biopsies -- 3.Proteomic Analysis -- 4.Western Blot Analysis -- 5.RT-PCR Analysis -- Acknowledgments -- References -- 5.Measurement of the Unfolded Protein Response (UPR) in Monocytes / Noel G. McElvaney -- 1.Introduction -- 2.Investigating UPR Activation in the Monocyte -- Acknowledgments -- References -- 6.Assessment of Endurance Exercise Training in Hepatic Endoplasmic Reticulum Stress / Natalie Ann Chapados -- 1.Introduction -- 2.Assessment of an Endurance-Training Program -- 3."Dietary-Inducible" Hepatic ER Stress -- 4.Assessment of Hepatic ER Stress Molecular Markers -- 5.Required Materials -- Acknowledgments -- References -- 7.Discovery Approaches to UPR in Athero-Susceptible Endothelium In Vivo / Peter F. Davies -- 1.Introduction -- 2.Procedures for the Isolation of Endothelial Cells and Preparation of RNA for Microarray Hybridization -- 3.Microarray Hybridization and Feature Extraction -- 4.Bioinformatics Analysis -- 5.Another Approach to Gene Connectivity: Weighted Gene Coexpression Network Analysis -- 6.Validation and Follow-up -- Acknowledgments -- References -- 8.Assessment of Endoplasmic Reticulum Stress and the Unfolded Protein Response in Endothelial Cells / Sven Horke -- 1.Introduction -- 2.The Acute Phase of ER Stress -- 3.The Adaptive Reaction Phase: Unfolded Protein Response in Endothelial Cells -- 4.The Final Phase: Cell Death Signaling -- Acknowledgments -- References -- 9.Measuring the Impact of Cigarette Smoke on the UPR / Ellen D. Jorgensen -- 1.Introduction -- 2.Preparing Samples for Analysis -- 3.Analysis of Activation of the PERK Pathway -- 4.ATF6 Pathway -- 5.IRE1 Pathway -- 6.Using LSC to Measure UPR Activation -- Acknowledgments -- References -- 10.Induction and Measurement of UPR and Osmotic Stress in the Yeast Pichia pastoris / Brigitte Gasser -- 1.Introduction -- 2.Measuring UPR Induction in P. pastoris -- 3.Inducing and Measuring Osmotic Stress in P. pastoris -- Acknowledgments -- References -- 11.Induction of ER Stress Response Leading to Programmed Cell Death in Trypanosoma brucei / Shulamit Michaeli -- 1.Introduction -- 2.Induction of ER Stress in Trypanosomes -- 3.Measurements of ER Stress in Trypanosomes Cells -- 4.Measurements of ER Stress-induced Cell Death -- Acknowledgments -- References -- 12.Measurement of Activation of the Endoplasmic Reticulum Stress Response in Autoimmune Myositis / Kanneboyina Nagaraju -- 1.Introduction -- 2.Measuring Endoplasmic Reticulum Stress Response in Skeletal Muscle Tissues Using Western Blotting -- 3.Immunohistochemical Detection of ER Stress Markers in Muscle Tissues -- 4.Determination of mRNA Levels of ER Stress Targets -- 5.Concluding Remarks -- Acknowledgments -- References -- 13.Activation of the Unfolded Protein Response in Human Acute Myeloid Leukemia / Thomas Pabst -- 1.Introduction -- 2.Technical Aspects -- 3.Discussion -- References -- 14.Modulation of the Unfolded Protein Response by GRP78 in Prostate Cancer / Salvatore V. Pizzo -- 1.Introduction -- 2.Methods for Monitoring UPR Modulation -- 3.Conclusions -- References -- 15.Endoplasmic Reticulum Stress in Brain Damage / Suresh L. Mehta -- 1.Introduction -- 2.ER Stress and Unfolded Protein Response -- 3.Cross Talk Between ER and Mitochondria -- 4.Experimental Approaches for the Detection of ER Stress -- Acknowledgment -- References -- 16.Methods and Models for Monitoring UPR-Associated Macrophage Death During Advanced Atherosclerosis / Edward B. Thorp -- 1.Introduction to ER Stress in Atherosclerosis -- 2.Significance of Macrophage ER Stress and Apoptosis in Advanced Atherosclerosis -- 3.Modeling Atherosclerosis-Relevant ER Stress-Induced Apoptosis -- 4.The UPR and Macrophage Death at the Murine Aortic Root -- 5.Conclusion and Future Methodological Advances -- Acknowledgments -- References -- 17.Detecting Autophagy in Response to ER Stress Signals in Cancer / Guillermo Velasco -- 1.Introduction -- 2.Monitoring Autophagy Upon ER Stress -- 3.Monitoring Autophagy Upon ER Stress In Vivo -- References -- 18.The Unfolded Protein Response Induced by Salt Stress in Arabidopsis / Ming Yuan -- 1.Introduction -- 2.The Treatment of Arabidopsis Seedlings -- 3.The Analysis of Unfolded Protein Response -- Acknowledgments -- References -- 19.Measurement of ER Stress Response and Inflammation in the Mouse Model of Nonalcoholic Fatty Liver Disease / Kezhong Zhang -- 1.Introduction -- 2.Methods to Measure ER Stress Response and Hepatic Inflammation in the Mouse Model of NAFLD -- 3.Visualizing ER Stress Response Associated with Liver Pathology in the Mouse Model of NAFLD -- Acknowledgments -- References.
- Subject(s)
- ISBN
- 9780123849915
0123849918 - Note
- Title from PDF title page (ScienceDirect, viewed Oct. 4, 2010).
AVAILABLE ONLINE TO AUTHORIZED PSU USERS. - Bibliography Note
- Includes bibliographical references and indexes.
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