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Functional glycomics [electronic resource] / edited by Minoru Fukuda

Published
Amsterdam, Netherlands ; Boston, Mass. : Elsevier/ Academic Press, [2010]
Copyright Date
©2010
Edition
1st ed.
Physical Description
xlviii, 447 pages, 16 unnumbered pages of plates : illustrations
Additional Creators
Fukuda, Minoru, 1945-
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Contents
Machine generated contents note: Section I Stem Cell -- 1.β1, 4-Galactosyltransferase V: A Growth Regulator in Glioma / Jianxin Gu -- 1.Overview -- 2.Experimental -- 3.Results -- 4.Conclusion and Future Direction -- Acknowledgments -- References -- 2.Roles of Polysialic Acid in Migration and Differentiation of Neural Stem Cells / Minoru Fukuda -- 1.Overview -- Acknowledgments -- References -- 3.Structural and Functional Analysis of Chondroitin Sulfate Proteoglycans in the Neural Stem Cell Niche / Andreas Faissner -- 1.Overview -- 2.Immunohistochemistry of Embryonic and Postnatal Mouse Brain Sections -- 3.Method to Stain Embryonic Sections for NSPC Markers -- 4.Analysis of the Adult Neurogenic Niche and SVZ-Derived Cells by Immunocytochemistry -- 5.Immunocytochemistry of Acutely Dissociated Cells -- 6.Isolation of NSPCs by Immunopanning or by Immunoisolation Using Paramagnetic Beads (EasySep) -- 7.Neurosphere Cultures and Various Methods for Their Analysis -- 8.Disaccharide Analysis of CS/DS Chains from Embryonic Brain and Conditioned Neurosphere Culture Media and Effect of Sodium Chlorate Treatment on Neurosphere Formation at Clonal Cell Density -- 9.Analysis of NSPC-Proliferation in Vitro and In Vivo -- 10.Analysis of CSPG Functions in NSPCs Using Chondroitinase ABC Treatment in Culture -- 11.Analysis of Chondroitin Sulfate Functions in the Neural Stem Cell Niche -- 12.RT-PCR and Semiquantitative Analysis of the Synthetic Machinery for Glycosaminoglycans -- 13.In Situ Hybridization of Sulfotransferases in Tissue and Neurosphere Sections -- 14.Microscopy -- 15.Conclusion and Outlook -- Acknowledgments -- References -- 4.Transcript Analysis of Stem Cells / Kelley W. Moremen -- 1.Introduction -- 2.qRT-PCR as a Tool for Determining Transcript Analysis for Glycan-Related Gene Expression -- 3.Examples of the Applications of qRT-PCR Transcript Analysis to Investigate Changes in Glycan-Related Gene Expression in Stem Cells -- Acknowledgments -- References -- 5.Directing Stem Cell Trafficking via GPS / Robert Sackstein -- 1.Overview -- 2.Rationale for GPS -- 3.Guiding Principles and Method for GPS -- 4.Method of α(1, 3)-Fucosylation of Cell Surface Using FTVI -- 5.Detection of E-Selectin Ligand Expression Following GPS -- 6.Testing for E-Selectin Ligand Activity Using E-Selectin-Ig Chimera (Three-Step Method for Flow Cytometry) -- 7.Summary -- Acknowledgments -- References -- 6.Functional Assays for the Molecular Chaperone Cosmc / Richard D. Cummings -- 1.Overview -- 2.T-Synthase and Cosmc -- 3.T-Synthase Activity Assay -- 4.Assay Activity for Function of Cosmc -- 5.Conclusion and Future Directions -- Acknowledgments -- References -- 7.Core 3-Derived O-Glycans Are Essential for Intestinal Mucus Barrier Function / Lijun Xia -- 1.Introduction -- 2.Generation of C3GnT-/- Mice -- 3.Disruption of the C3GnT Gene Eliminates Core 3-Derived O-Glycans and Exposes the Tn Antigen in Murine Colon -- 4.Deficiency of C3GnT Results in Reduced Muc2 Expression in Colon and Impaired Mucosal Integrity -- 5.C3GnT-/- Mice are Highly Susceptible to Dextran Sodium Sulfate-Induced Colitis -- 6.Conclusion and Future Direction -- Acknowledgments -- References -- 8.Core3 Glycan as Tumor Suppressor / Minoru Fukuda -- 1.Overview -- 2.Generating Core3 Glycan Expressing Cell Lines -- 3.Detection Methods of Core3 Expression -- 4.Migration Assay Using Attractant -- 5.Determination of Major Integrin Using Functional Blocking Antibodies -- 6.Tumor Formation Assay -- 7.Western Blotting and Lectin Blotting -- 8.Heterodimerization Assay -- 9.FAK Signaling -- References -- 9.Characterization of Mice with Targeted Deletion of the Gene Encoding Core 2 β1, 6-N-Acetylglucosaminyltransferase-2 / Minoru Fukuda -- 1.Introduction -- 2.Generation and Genotyping of C2GnT2 KO Mice -- 3.Mice Lacking C2GnT2 Have Reduced Core 2 and no Core 4 Enzyme Activity and Altered Glycosylation -- 4.Phenotyping C2GnT2 KO Mice -- 5.Conclusions and Future Directions -- Acknowledgments -- References -- 10.Analyzing Physiological Function of Polypeptide GalNAcT-1-Deficient Mice in Humoral Immunity / Mari Tenno -- 1.Overview -- 2.Assay for In Vitro B Lymphocyte Activation -- 3.Assay for In Vivo Antibody Production -- 4.Immunohistochemical Staining of Frozen Sections -- 5.Apoptosis Detection Using Antibody Against Caspase 3 Active Form -- 6.Apoptosis Detection by TUNEL System -- Acknowledgment -- References -- 11.β3GnT2 (B3GNT2), A Major Polylactosamine Synthase: Analysis of B3gnt2-Deficient Mice / Hisashi Narimatsu -- 1.Overview -- 2.Glycogenes for Polylactosamine Synthesis (i.e., β1, 3-N-Acetylglucosaminyltransferase Genes) -- 3.N-Glycan Polylactosamine is Greatly Reduced in B3gnt2-/- Mice -- 4.Phenotype of B3gnt2-/- Lymphocytes Lacking Polylactosamine on N-Glycans -- 5.Protocols -- Acknowledgments -- References -- 12.Targeted Genetic Inactivation of N-Acetylglucosaminyltransferase-IVa Impairs Insulin Secretion from Pancreatic β Cells and Evokes Type 2 Diabetes / Kazuaki Ohtsubo -- 1.Overview -- 2.Engineering GnT-IVa-Deficient Mice -- 3.GnT-IV Enzymology -- 4.Glucose and Insulin Homeostasis -- 5.Immunohistochemical Analysis -- 6.Islet Cell Preparation and Culture -- 7.Pulse-Chase Labeling -- 8.Cell Surface Half-Life Time of GLUT2 -- 9.GLUT2 Glycan Analysis by Lectin Blot -- 10.Cell-Surface Protein Cross-Linking -- Acknowledgment -- References -- 13.The Ashwell-Morell Receptor / Prabhjit K. Grewal -- 1.Overview -- 2.Endogenous Ligands of the AMR -- 3.Hepatocytes in Molecular Clearance Mechanisms of the Liver -- 4.Genotyping HL-1-or HL-2-Deficient Mice -- 5.Hematology and Coagulation Analyses Methods (Partly Adapted from Ellies et al., 2002; Grewal et al., 2008; Wang et al., 2001) -- Acknowledgments -- References -- 14.Roles of GlcNAc-6-0-Sulfotransferases in Lymphoid and Nonlymphoid Tissues / Hiroto Kawashima -- 1.Overview -- 2.Establishment of a Mouse Colon Adherent Cell Line and Cell Culture -- 3.Treatment of CAdC1 Cells with SCFAs -- 4.RT-PCR -- 5.Histology and Immunostaining -- 6.Preparation of Colonic-Mucin-Enriched Fraction -- 7.Carbohydrate Structural Analysis -- 8.Induction of Colitis by Dextran Sulfate Sodium -- Acknowledgments -- References -- 15.Core O-Glycans Required for Lymphocyte Homing: Gene Knockout Mice of Core 1 β1, 3-N-Acetylglucosaminyltransferase and Core 2 N-Acetylgtucosaminyltransferase / Minoru Fukuda -- 1.Introduction -- 2.Lymphocyte Homing Assay -- 3.Staining of Lymph Nodes by L- and E-Selectin-IgM Chimeric Proteins after Glycosidase Treatment -- 4.Probing of L-Selectin Ligands on Membrane Filters with L- and E-selectin-IgM Chimeric Proteins -- 5.Conclusions -- Acknowledgments -- References -- 16.Immunohistochemical Analysis of Carbohydrate Antigens in Chronic Inflammatory Gastrointestinal Diseases / Jun Nakayama -- 1.Overview -- 2.Immunohistochemical Analysis Using Conventional Immunostaining -- 3.Immunohistochemical Analysis Using Multiple Immunostaining -- 4.Immunohistochemical Analysis Using L-Selectin-IgM Chimera Binding -- Acknowledgments -- References -- 17.Genetic Defects in Muscular Dystrophy / Paul T. Martin -- 1.Overview -- 2.Mouse Models of Muscular Dystrophy -- 3.Approach to Phenotype Analysis in Mouse Muscular Dystrophy Models -- 4.Summary -- Acknowledgments -- References -- 18.POMT1 is Essential for Protein O-Mannosylation in Mammals / Sabine Strahl -- 1.Overview -- 2.Experimental -- 3.Discussion/Conclusions -- Acknowledgments -- References -- 19.POMGnT1, POMT1, and POMT2 Mutations in Congenital Muscular Dystrophies / Pascale Guicheney -- 1.Overview -- 2.Methods -- 3.Procedures for Enzymatic Activity and Mutation Search -- Acknowledgment -- References -- 20.Cellular and Molecular Characterization of Abnormal Brain Development in Protein O-Mannose N-Acetylglucosaminyltransferase 1 Knockout Mice / Huaiyu Hu -- 1.Overview -- 2.Analysis of α-DG Glycosylation and Laminin Binding by Western Blot -- 3.Histological Analysis of POMGnT1 Knockout Brain -- 4.Lamination Defects in the Neocortex of POMGnT1 Knockout Mice -- 5.Analysis of the Pial Basement Membrane by Laminin Immunostaining -- 6.Analysis of the Pial Basement Membrane by Transmission Electron Microscopy -- 7.Analysis of the Glia Limitans by GFAP Immunofluorescence Staining -- Acknowledgment -- References -- 21.Investigating the Functions of LARGE: Lessons from Mutant Mice / Jane E. Hewitt -- 1.Overview -- 2.Human LARGE and Relevance to Disease -- 3.Identification of Mice Carrying Mutations in Large -- 4.Loss of Functional Large Protein Results in Hypoglycosylation of α-Dystroglycan -- 5.Phenotypes of Mice with Mutations in Large -- 6.Expression of LARGE Genes -- 7.Does Large Encode a Functional Glycosyltransferase? -- 8.Largemyd Mice as a Model for Therapeutic Approaches to Dystroglycanopathy -- Acknowledgments -- References -- 22.A Tumor Suppressor Function of Laminin-Binding α-Dystroglycan / Minoru Fukuda -- 1.Background -- 2.Methods -- Acknowledgment -- References -- 23.Tumor Formation Assays / Chikara Ohyama -- 1.Overview -- 2.Animal Care and Protocol Approval -- 3.Disinfection of Mice -- 4.Analgesia and Anesthesia in Mice -- 5.IP Injection -- 6.IV Injection into the Tail Vein -- 7.IV Tumor Formation Assay Using Immune-Deficiency Mice -- 8.SC Inoculation -- 9.FP Inoculation -- 10.Testicular Inoculation -- 11.Prostate Inoculation -- Acknowledgment -- References.
Subject(s)
Genre(s)
ISBN
9780123809971
0123809975
Bibliography Note
Includes bibliographical references and indexes.
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