Test No. 455: The Stably Transfected Human Estrogen Receptor-alpha Transcriptional Activation Assay for Detection of Estrogenic Agonist-Activity of Chemicals [electronic resource] / Organisation for Economic Co-operation and Development
- Corporate Author:
- Organisation for Economic Co-operation and Development
- Paris : OECD Publishing, 2009.
- Physical Description:
- 16 p.
- OECD Guidelines for the Testing of Chemicals, Section 4, 2074-5788
- Restrictions on Access:
- License restrictions may limit access.
- This Test Guideline describes an in vitro assay, which provides mechanistical information, and can be used for screening and prioritization purposes. The test system utilises the hERalpha-HeLa-9903 cell line derived from a human cervical tumor and stably transfected. This cell line can measure the ability of a test chemical to induce hERalpha-mediated transactivation of luciferase gene expression. The cells are exposed to 7 non-cytotoxic concentrations of the test chemical for 20-24 hours to induce the reporter gene products. Four reference chemicals should be included in each experiment: a strong estrogen (17beta-estradiol), a weak estrogen (17alpha-estradiol), a very weak estrogen (17alpha-methyltestosterone) and a negative control (corticosterone). The activity of the luciferase enzyme is measured in a luminometer. A test chemical is considered to be positive if the maximum response induced is equal to or exceeds 10% of the response of the positive control (1 nM 17alpha-estradiol) in at least two of two or two of three runs.Software to be used with TG 425, 432, 455. Click here. Software not part of the Mutual Acceptance of Data.
- Other Edition:
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