RNAi and microRNA-mediated gene regulation in stem cells [electronic resource] : methods, protocols, and applications / edited by Baohong Zhang and Edmund J. Stellwag
- Published
- New York : Humana Press, c2010.
- Physical Description
- xi, 261 p. : ill. (some col.) ; 27 cm.
- Additional Creators
- Zhang, Baohong and Stellwag, Edmund J. (Edmund Johannes), 1951-
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- Contents
- RNAi in stem cells : current status and future perspectives / Gang-Ming Zou -- A brief introduction to RNAi and microRNAs in stem cells / Alexander K. Murashov -- Forward RNAi screens in human stem cells / Christine Karlsson, Jonas Larsson, and Aurélie Baudet -- A recessive genetic screen for components of the RNA interference pathway in mouse embryonic stem cells / Melanie I Trombly and Xiaozhong Wang -- Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian -- Establishing efficient siRNA knockdown in stem cells using fluorescent oligonucleotides / Stephen W. Chen and Steve K.W. Oh -- Loss-of-function studies in mouse embryonic stem cells using the pHYPER shRNA plasmic vector / Soizik Berlivet, Martin Houlard, and Matthieu Gérard -- Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka -- Silencing of rho-GDI[gamma] by RNAi promotes the differentiation of neural stem cells / Jiao Wang, Wei Lu, and Tieqiao Wen -- RNAi knockdown of redox signaling protein Ape1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Cynthia LeBron, and Yumei Fu -- Proteins involved in cell migration from glioblastoma neurospheres analyzed by overexpression and siRNA-mediated knock-down / Carsten Hagemann ... [et al.] -- An efficient transfection method for mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang -- Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection / Mikako Saito and Hideaki Matsuoka -- Preparation and analysis of microRNA libraries using the Illumiina massively parallel sequencing technology / Ryan D. Morin ... [et al.] -- Assessing in vivo microRNA function in the germline stem cells of the Drosophila ovary / Kin Chan and Hannele Ruohola-Baker -- Monitoring microRNA expression during embryonic stem-cell differentiation using quantitative real-time PCR (qRT-PCR) / Xiaoping Pan ... [et al.] -- Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison -- Efficient gene knockdowns in mouse embryonic stem cells using microRNA-based shRNAs / Jianlong Wang.
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- ISBN
- 9781607617686 (hbk. : alk. paper)
1607617684 (hbk. : alk. paper)
9781607617693 (e-ISBN)
1607617692 (e-ISBN) - Bibliography Note
- Includes bibliographical references and index.
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