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Plant tissue culture [electronic resource] : techniques and experiments / Roberta H. Smith

Author
Smith, Roberta H.
Published
[Place of publication not identified] : Academic Press, 2012.
Edition
3rd ed.
Physical Description
1 online resource
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Contents
Machine generated contents note: History of Plant Cell Culture / Trevor A. Thorpe -- Introduction -- The Early Years -- The Era of Techniques Development -- The Recent Past -- Cell Behavior -- Plant Modification and Improvement -- Pathogen-Free Plants and Germplasm Storage -- Clonal Propagation -- Product Formation -- The Present Era -- Setup of a Tissue Culture Laboratory -- Work Areas -- Media Preparation/Culture Evaluation/Record-Keeping Area -- Aseptic Transfer Area -- Environmentally Controlled Culture Area -- Media Components and Preparation -- Inorganic Salts -- Plant Growth Regulators -- Vitamins -- Carbohydrates -- Hexitols -- Gelling Agent -- Amino Acids -- Antibiotics -- Natural Complexes -- Media pH -- Medium Preparation -- Explant Preparation -- Explant Age -- Season -- Explant Size -- Plant Quality -- Goal -- Genotype -- Aseptic Technique -- Aseptic Germination of Seeds -- Carrot, Sunflower, Broccoli, Cotton, or Any Seed of Choice -- Douglas Fir -- Contamination -- Explant -- Disinfecting Agents -- Explant Source -- Internal Microbial Contamination -- Explant Pretreatment to Control Microbial Contaminants -- Personnel -- Laminar Air Flow Hood -- Insects -- Control of Insect Contamination -- Media -- Instruments -- Room Air Handling System -- Callus Induction -- Callus Initiation -- Explant Preparation -- Culture -- Observations -- Data and Questions -- Explant Orientation -- Explant -- Observations -- Establishment of Competent Cereal Cell Cultures -- Explants -- Data and Questions -- Salt Selection In Vitro -- Explants -- Data and Questions -- Growth Curves -- Cotton -- Tobacco -- Cellular Variation from Callus Cultures -- Explant -- Data and Questions -- Regeneration and Morphogenesis -- Controlled Morphogenesis: Potato Tuberization -- Explant -- Data and Questions -- Somatic Embryogenesis -- Explant Preparation -- Procedure -- Data and Questions -- Regeneration of Rice -- Regeneration -- Data and Questions -- Dormancy Requirements of Explants -- Explant Preparation -- Questions -- Primary Morphogenesis: Douglas Fir -- Explant Preparation -- Data and Questions -- Woody Shrubs and Trees / Brent H. McCown -- Rhododendrons and Azaleas -- Explants -- Advanced Studies -- Roses -- Explant -- Advanced Studies -- Birch Trees -- Explant -- Advanced Studies -- White Cedar (Arborvitae) -- Explant -- Haploid Plants from Anther Culture -- Datura Anther Culture -- Explant Preparation -- Questions -- African Violet -- Explant Preparation -- Observations -- Root Tip Chromosome Squash Technique -- Questions -- Tobacco -- Explant Preparation -- Observations -- Questions -- Anther Squash Technique -- Embryo Rescue -- Sweet Corn Embryo Culture -- Preparation and Culture of Corn Embryos -- Questions -- Crabapple and Pear -- Explant Preparation -- Observations -- Questions -- Meristem Culture for Virus-Free Plants -- Isolation of the Shoot Apical Meristem -- Explant Preparation -- Questions -- Establishing Virus- and Bacteria-Free Plants -- Explant Preparation -- Garlic Propagation from Bulb Scales -- Explant Preparation -- Data -- In Vitro Propagation for Commercial Production of Ornamentals -- Boston Fern -- Stage I -- Stage II -- Stage III -- Staghorn Fern -- Stage I -- Stage II -- Stage III -- Ficus -- Stage I -- Stage II -- Stage III -- Kalanchoe -- Explant Preparation -- Data and Questions -- African Violet -- Explant Preparation -- Data and Questions -- Endangered Species -- Pitcher Plants -- Cactus -- Explant Preparation -- Protoplast Isolation and Fusion / James E. Craven -- Plant Protoplast Isolation and Fusion -- Supplies -- Protoplast Liberation -- Protoplast Purification -- Vital Staining -- Protoplast Fusion -- Yield Determination -- Developing a Protoplast Isolation Protocol -- Agrobacterium-Mediated Transformation of Plants / Stuart Sprague -- Petunia or Tobacco Leaf Disk -- Sequence -- Preculture -- Explant Preparation -- Cocultivation with Agrobacterium -- Shoot Transformation and Elimination of Agrobacterium -- Rooting and Pretesting for Transformed Shoots -- Petunia Shoot Apex -- Sequence -- Seed Germination -- Shoot Induction -- Shoot Apex Transformation -- Rooting -- Tobacco Leaf Infiltration -- Sequence -- Grow Agrobacterium -- Culture Agrobacterium -- Harvest Agrobacterium -- Infiltration Tobacco Leaves.
Summary
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature .
Subject(s)
Genre(s)
ISBN
9780124159204 (electronic bk.)
0124159206 (electronic bk.)
Note
Includes index.
AVAILABLE ONLINE TO AUTHORIZED PSU USERS.
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